The settings are almost irrelevant for this type of image, guys. Yeah, you need a setting so that you actually can see what you are imaging, so lighting, f stop, and focus are important as always, but the secret to this type of image lies in a freeware stacking programme called CombineZP. I am using the earlier ZM version.
I start with making a fairly robust and smooth-sided cradle for the camera. I have been using the points filing tools from Fast Tracks as a side and backstop against which I can slide the camera after I trip the shutter and before the timer opens it. The secret, and the time, goes into setting up the cradle so that your stack of images, each image, has almost precisely the same aiming point relative to the scene being imaged. This is important! Sloppy re-aiming between shots means lots of garbage in the product rendered by CombineZM.
I start with the macro setting, get two or three images with focus at 1.5", then 2.5", and lastly 7" from the camera. Then I switch out of macro mode, take another 3 images with another 7" or so of depth from the lens, and the last two or three of the stack (totaling about 8-10 images in all) cover the depth needed to get to the backdrop.
Just to be clear, one image per distance of focus, and each one must be a succession with added depth of field...that's what the software wants, a series of successive (in order) images from which to draw all the sharpest details in each.
Next step is to do a batch conversion of all your raw or jpeg images into tiff files so that they all have the same file density/size. Also a requirement by CZM. Once done, you open CZM, import those tiff files, and the rest I'd have to explain as we go along if you are interested.
The results can be stunning if the usual care is taken to frame the subject, clean up the scene, arrange things so that they look interesting and natural, etc. The software will show defects or deficiencies up close and personal, so just a word to the wise...you'll have to take steps to dust, remove upturned sow bugs, mouse poop, or whatever, and finish weathering jobs that may be less than optimal for close viewing...you get the idea.
-Crandell